J Dermatol Plast Surg | Volume 5, Issue 1 | Research Article | Open Access

Gene Expression Change Analysis Following Specific Comprehensive Solar Protection SPF50+ after Ultraviolet Light and Blue Light Exposure in a 3- Dimensional Reconstructed Human Skin Model

Tanaka Y^1*, Parker R² and Aganahi A²

¹Clinica Tanaka Plastic, Reconstructive Surgery and Anti-aging Center, Japan ²RATIONALE, Australia

^*Correspondance to: Yohei Tanaka 

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Abstract

Background: Clinical, optical and histological studies report that solar damage continues to pose a threat to skin health despite sunscreen usage and sun awareness campaigns. Purpose: To investigate gene expression changes following topical solar protection after ultraviolet and blue light exposure. Methods: Using epidermal keratinocytes and dermal fibroblasts derived from a 3-dimensional reconstructed human skin model, gene expression was assessed via the Genemarkers Standard Skin Panel deploying DNA microarray and quantitative real-time PCR exploration. All of the tissue samples were then inoculated with the solar protection formulation capable of blocking solar energy from ultraviolet, visible light (including blue light) and near-infrared energy (#3 The tinted Serum SPF50+, RATIONALE, Victoria, Australia). Results: A DNA microarray analysis revealed 13 genes that were dramatically up-regulated by at least two-fold out of 107 genes after ultraviolet exposure, and 12 genes that were dramatically upregulated by at least two-fold out of 52 genes after blue light exposure. The quantitative real-time PCR revealed that, in comparison to the control, the genes encoding Toll Like Receptor 5 (TLR5), Metallothionein 1G (MT1G), Tumor Protein 73 (TP73), Filaggrin (FLG), and Caspase 14 (CASP14) were up-regulated after ultraviolet light exposure, 4.57, 2533.59, 4.94, 13.46 and 2.13, respectively. Lecithin Retinol Acyltransferase (LRAT), Sequestosome 1 (SQSTM1), Loricrin (LOR), RAR Related Orphan Receptor A (RORA) were up-regulated after blue light exposure, 6.07, 2.3, 51.82, and 3.56, respectively. Conclusion: This study demonstrates that the specific comprehensive solar protection upregulated genes involved in photoprotection and anti-photoaging mechanisms in a 3-dimensional reconstructed human skin model.

Keywords:

Gene expression; Anti-photoaging; Antioxidant; Photoprotection; Barrier repair; DNA repair; Hydration; Near-infrared

Citation:

Tanaka Y, Parker R, Aganahi A. Gene Expression Change Analysis Following Specific Comprehensive Solar Protection SPF50 after Ultraviolet Light and Blue Light Exposure in a 3-Dimensional Reconstructed Human Skin Model. J Dermatol Plast Surg. 2024; 5(1): 1026..