J Dent Oral Biol | Volume 8, Issue 2 | Research Article | Open Access

Biomechanically Induced Expression of Wnt5a and Ror2 Plays a Detrimental Role in Degenerative Remodeling of the Rat Temporomandibular Joint Induced by Abnormal Occlusion

Chen L^1*, Kuang B1*, Bu Y¹, Han X², Qi M¹, Jiang W¹ and Sun Y^1*

¹Department of Stomatology, First People's Hospital, China
²State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi
Key Laboratory of Stomatology, The Fourth Military Medical University, China

^*Correspondance to: Yanyan Sun 

Fulltext PDF

Abstract

The present study aimed to test whether abnormal mechanical loading could increase expression of Wnt5a and Ror2 and their effects on the degenerative changes in rat temporomandibular joints. Primary chondrocytes and bone marrow stromal cells isolated from rat condylar cartilage and subchondral bone were stimulated using fluid flow shear stress, and pretreated with STAT3, NFkappaB, and Notch pathway inhibitors. The gene and protein levels of Wnt5a and Ror2 were tested. The inhibitor of Wnt5a-Ror2 signaling was injected periarticularly into the temporomandibular joint of rats showing degenerative changes induced by abnormal molar occlusion. The effects of sRor2 on cartilage and subchondral bone pathology were evaluated. In chondrocytes, Wnt5A and Ror2 expression increased and peaked at 120 min or 30 dynes/cm2 of fluid flow shear stress, while those in bone marrow stromal cells peaked at 60 min or 20 dynes/cm2 of FSS (P<0.05). Fluid flow shear stress-induced Wnt5a expression in chondrocytes and bone marrow stromal cells was regulated by the STAT3 pathway. The fluid flow shear stress induced expression of Ror2 was regulated by STAT3 and Notch in chondrocytes and by NF-kappaB and Notch in bone marrow stromal cells. Progressive cartilage degenerative changes and subchondral bone loss of rat temporomandibular joint were markedly reversed by 1 μg/week sRor2.

Keywords:

Mechanical loading; Temporomandibular joint; Osteoarthritis; Wnt5A; Ror2

Citation:

Citation: Chen L, Kuang B, Bu Y, Han X, Qi M, Jiang W, et al. Biomechanically Induced Expression of Wnt5a and Ror2 Plays a Detrimental Role in Degenerative Remodeling of the Rat Temporomandibular Joint Induced by Abnormal Occlusion. J Dent Oral Biol. 2023; 8(2): 1214. ISSN: 2475-5680.