J Dent Oral Biol | Volume 8, Issue 2 | Research Article | Open Access

A Novel Approach for Instant Quantification of Bacterial Load During Root Canal Therapy Using Quantitative Light Induced Fluorescence - A Pilot Study

Nandlal B*, Raghavendra S, Aiswarya B and Shreya K

Department of Pediatrics and Preventive Dentistry, JSS Dental College, India

*Correspondance to: Nandlal B 

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Abstract

Background: Root canal treatments are undertaken to eradicate bacterial infections from root canals and retain the natural tooth. The persistence of microorganisms in the root canal space even after chemo-mechanical preparation is one of the common reasons for root canal failure. The current methods to evaluate the presence of bacteria after therapy is not widely practiced because of the lack of a rapid chairside method. The present study demonstrates the use of QLF in this field. Methods: Root canal sample was collected from the patient’s infected tooth with a history of pain and swelling. Exudate samples were taken using sterile paper points after access opening and extirpation in the canal (S1) and after biomechanical preparation and irrigation (S2). Intracanal medicament was given and recalled after one week, again exudate samples were collected (S3). QLF images were obtained with QLF-D Biluminator™ for all samples. Result: The analysis was done using QA3 version 1.26 Software. The extent of redness in the blue light image depicted the bacterial load instantly and the analysis depicted the reduction of the Mean Bacterial Load based on SPS scoring and ΔR values objectively. Conclusion: It is possible to state that QLF is a highly precise and reliable tool for the instant prediction of bacterial load in the root canal system based on the presence of porphyrins. Our concept of QLF in endodontics instantly help us to visualize the bacterial load before and after treatment.

Keywords:

QLF; Endodontic therapy; Rapid chairside method; Bacterial load

Citation:

Nandlal B, Raghavendra S, Aiswarya B, Shreya K. A Novel Approach for Instant Quantification of Bacterial Load During Root Canal Therapy Using Quantitative Light Induced Fluorescence - A Pilot Study. J Dent Oral Biol. 2023; 8(2): 1212..

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