J Dent Oral Biol | Volume 8, Issue 1 | Research Article | Open Access

Immunohistochemical Distributions of Fibroblast Growth Factor 1 and 2 in the Mouse Central Nervous System in Different Time Course

Rashid ME^1,2* and Akhter K^3,4

¹Department of Oral Growth and Developmental Biology, Hiroshima University Graduate School of Dentistry, Japan
²Department of Oral Basic and Clinical Sciences, College of Dentistry, Taibah University, KSA
³Department of Oral and Maxillofacial Surgery, Division of Cervico-Gnathostomatology, Hiroshima University
Graduate School of Dentistry, Japan
⁴Department of Periodontology and Oral Pathology, Pioneer Dental College and Hospital, University of Dhaka,
Bangladesh

^*Correspondance to: Mohammed Enamur Rashid 

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Abstract

Fibroblast Growth Factor 1 (FGF1) and FGF2 are involved in the development of the central nervous system. We evaluated the spatiotemporal distributions of FGF1 and FGF2 in the ICR male mouse cerebrum from Postnatal day 0 (P0) to P360. Western blot analysis of whole cerebrum extracts confirmed that FGF2 gradually increased during P10 to P90 and thereafter reached a plateau. FGF2 was detected as early as P0 and invariable throughout the study period. Cryosections of the cerebrums were subjected to immunohistochemistry, to demonstrate the localization of FGF1 and FGF2. The study has detected the time-course of the appearance of FGF1 and FGF2 immunoreactivity in the mouse brain. The occurrence of active neurite outgrowth, synapse formation, and sprouting was identified including eyelids opening, Corti’s organ, terminal differentiation, and initiation of myelination.

Keywords:

Central nervous system; Fibroblast growth factors; Immunohistochemistry

Citation:

Rashid ME, Akhter K. Immunohistochemical Distributions of Fibroblast Growth Factor 1 and 2 in the Mouse Central Nervous System in Different Time Course. J Dent Oral Biol. 2023; 8(1): 1206. ISSN: 2475-5680.