Ann Infect Dis Epidemiol | Volume 1, Issue 1 | Case Report | Open Access
David A Baker*, Tatyana Peresleni and Kocis Christina
Department of Obstetrics, Gynecology and Reproductive Medicine, Stony Brook University, USA
*Correspondance to: David A Baker
Fulltext PDFObjective: The objective of this study was to investigate the cytokines secretion in patients with vestibulodynia which affects up to 16% of U.S. women. Our main goal was to find out which cytokines may serve as biological markers of this debilitating condition. Study
Design: The cytokines expression profiles in 32 patients with vestibulodynia and 26 healthy volunteers were evaluated. The vaginal rinses of all participants were analyzed for the secretion of 40 cytokines using the semi-quantitative Ray-Bio Human Inflammation Antibody Arrays, followed by densitometry and statistical analyses.
Results: IL-8 was significantly increased in vestibulodynia patients compared to controls. IL-8 was the only pro-inflammatory cytokine to be up-regulated significantly. Interleukins GM-CFS, MCSF, and IL-10 were elevated in vestibulodynia patients, although not significantly. IL-12p40 and IL- 12p70 were minimally elevated. Interleukins IL-1β, RANTES, IL-2, IL-15, TGF-δ1, IL-16, TNF- α, IL-4, IL-17, IP-10,EOTAXIN-1 and -2, IL-6, IL-6sR, MCP-1, G-CFS, TIMP-2, PDGF-BB, I-309, MIP-1β, IL-1α, MIP-1δ were significantly down regulated in vestibulodynia patients vs. controls. Conclusions: These findings show that IL-8, with its ability to activate neutrophil granulocytes, emerged as an undoubted marker in vestibulodynia. MCSF and GM-CFS may act together with IL- 8, stimulating the production of macrophages and dendritic cells and taking part in inflammation and pain development. However, whole group of cytokines and chemokines was down-regulated in vestibulodynia patients. All of the above suggests that vestibulodynia appears to be a result of non-classical cytokine-mediated inflammatory and pain syndrome, where IL-8 appears to be the prominent marker on inflammation and pain syndrome.
Baker DA, Peresleni T, Christina K. Inflammatory Markers in Vestibulodynia. Ann Infect Dis Epidemiol. 2016; 1(1): 1002.