Ann Clin Immunol MicroBiol | Volume 2, Issue 1 | Research Article | Open Access

Accuracy of Malaria Diagnosis by RDT using Microscopy as gold standard amongst Pregnant Women Attending Antenatal Clinic in Eket

Ekom Ndifreke Edem1*, Emem Okon Mbong2and Sajjad Hussain3

1Department of Medical Microbiology, University of Uyo Teaching Hospital, Nigeria
2Department of Environmental Biology, Heritage Polytechnic, Ikot Udoata, Nigeria
3Department of Microbiology, Harbin Medical University, China

*Correspondance to: Ekom Ndifreke Edem 

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The global impact of malaria has spurred interest in developing effective diagnosis strategies not only for resource on limited areas where malaria is a burden on society, but also in developed countries where malaria diagnostic expertise is lacking. Malaria diagnosis involves identifying malaria parasite or antigens product in patients’ blood. Although this may seem simple, the diagnostic accuracy is subject to many factors, including expertise, sensitivity and effectiveness of diagnostic tools utilized. This study was conducted to ascertain whether the use of microscopy and Rapid Diagnostic test (RDT) methods influences accuracy of malaria diagnosis on pregnant women attending antenatal clinic in Eket. Forty (40) consenting pregnant women were recruited in the study. Blood samples collected through venous procedure were analyzed microscopically and SD Bioline Malarial test kits. Socio-demographic data showed 50% were participants between the age of <18 – 34 years and 40% were ≥35years. Fourteen (35%) of the participants had tertiary education, 25% hadprimary and secondary education each and 15% had no formal education. Thirty (75%) samples werepositive to the Ab-Ag RDT test with 10 (25%) samples being negative, while 24 (60%) samples were positive to microscopy with 16 (40%). RDT had a 75% of sensitivity, 25% specificity, 60% PPV and 40% NPV. This
study revealed that the detection rate of malaria parasite by RDT is higher than microscopy and this could be because of some human factor during microscopy processes.


Microscopy; Rapid diagnostic test (RDT); Accuracy; Malaria; Pregnant


Edem EN, Mbong EO, Hussain S.Accuracy of Malaria Diagnosis by RDTusing Microscopy as gold standardamongst Pregnant Women AttendingAntenatal Clinic in Eket. Ann Clin Immunol Microbiol. 2020; 2(1): 1015..

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