Ann Clin Anat | Volume 3, Issue 1 | Research Article | Open Access

Sodium Nitrite-Induced Hypoxic Nephrotoxicity in Adult Rats

Zeenat F Zaidi*

Department of Anatomy, United Medical & Dental College, Pakistan

*Correspondance to: Zeenat Fatima Zaidi 

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Kidney hypoxia plays an important role in the pathogenesis of acute and chronic kidney disease and has pronounced effects on renal function. Ischemic-induced renal tissue hypoxia is thought to be a major component in the development of acute renal failure. The objective of this study was to explore the effects of chemically-induced hypoxia on renal structure.
Methodology: Adult male albino rats, weighing 180 gm to 200 gm were used in this study. The animals were fasted for three hours prior to subcutaneous injection of sodium nitrite (75 mg/kg). One hour after drug administration, rats were decapitated. The kidneys were removed and placed overnight in fixative containing 10% formalin. Paraffin-embedded kidney tissue blocks were cut serially into coronal slices of 5 μ thickness and stained with Hematoxylin and Eosin (H and E) staining. Morphological assessment of injury was determined in the cortex by counting the evidence of glomerular degeneration, tubular dilatation & necrosis, loss of brush border and renal congestion, from 10 high-power (X40) fields/rat.
Results: Numerous kidney glomeruli showed atrophy with dilatation of Bowman’s space. Many of the proximal convoluted tubules were dilated and showed epithelial desquamation, degeneration and necrosis. The distal convoluted tubules showed no changes.
Conclusion: Sodium nitrite induced hypoxia results in necrotic changes in the glomeruli and the proximal convoluted tubules in adult rats.


Hypoxia; Kidney; Sodium nitrite; Proximal convoluted tubules; Glomerulus; Tubular necrosis


Zaidi ZF. Sodium Nitrite-Induced Hypoxic Nephrotoxicity in Adult Rats. Ann Clin Anat. 2020;3(1):1009..

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