Yevgeny Tendler1, Michael Aviram1,2, Nina Volkova2 and Marielle Kaplan1,2*
1Clinical Biochemistry Laboratory, Rambam Medical Center, Israel
2Lipid Research Laboratory, Technion – Israel Institute of Technology, Israel
C-Reactive Protein (CRP) is a sensitive systemic marker of inflammation and was identified as a biomarker of cardiovascular diseases. Pathogenesis of atherosclerosis, an inflammatory disease, involves upregulation of oxidative mechanisms and paraoxonases which exhibit antioxidative activities have been shown to inhibit Atherogenesis. Extracellular vesicles such as exosomes and micro particles serve as containers of biological information on various pathophysiological settings. The goal of the present study was to analyze the possible presence of CRP in micro particles or in exosomes fractions isolated from serum samples and the possible effects of the serum CRP-rich, MPs or exosomes fractions on macrophage antioxidative properties such as PON2 activity. Serum exosomes, but not micro particles, exhibited high concentration of the inflammatory marker CRP. Moreover, serum exosome-rich fraction was shown to significantly increase the activity of macrophage PON2, an anti-atherogenic and antioxidative enzyme. In conclusion, the secretion of CRP through exosomes could lead to new directions in understanding the mechanism of action of CRP in inflammatory processes and serum exosomes could have anti-atherogenic and anti-bacterial properties.
endler Y, Aviram M, Volkova N, Kaplan M. Human Serum CRP – Rich Exosomes Fraction Increases the Activity of Macrophage Paraoxonase 2 (Pon2). Ann Atheroscler Res. 2018;1(1):1004.