Am J Clin Microbiol Antimicrob | Volume 2, Issue 1 | Research Article | Open Access
Yousuf ul Bashir1*, Nargis Bali2, Sarbjeet Sharma1, Tabindah Jahan3 and Huda Shafi4
1Department of Microbiology, Sri Guru Ram Das Institute of Medical Sciences and Research Amritsar, India
2Department of Microbiology, Shere-i-Kashmir Institute of Medical Sciences, India
3Department of Microbiology, Government Medical College Srinagar, India
4Government Dental College Srinagar, India
*Correspondance to: Yousuf ul Bashir
Fulltext PDFBackground: Detection of SCCmec types in strains of Methicillin Resistant Staphylococcus aureus (MRSA) can aid in tracing the origin and spread of these microorganisms. Aims: To find out the most common SCCmec types and subtypes in clinical isolates of S. aureus. Settings and design: This study was carried out at a tertiary care centre of North India for a period of one year. Material and
Methods: Staphylococcus aureus recovered by standard microbiological procedures form various clinical samples such as blood, urine, pus and other body fluids was subjected to antimicrobial susceptibility testing along with multiplex PCR to detect various SCCmec types and subtypes. Statistical Analysis: Fisher’s exact test was done to determine the statistical significance. A P-value of <0.05 was considered as statistically significant.
Results: A total of 400 isolates of Staphylococcus aureus (200 from in-patient department, IPD and out-patient department, OPD each) were recovered out of which 55.5% from IPD and 32.5% from OPD were resistant to methicillin. Maximum isolation of Methicillin Resistant Staphylococcus aureus (MRSA) was from pus samples and in patients in the age group of 41 years to 50 years. SCCmec types III, IVc, IVa and V were seen in MRSA isolated from the IPD and SCCmec types IVa, IVc and V were seen in those recovered from OPD. Significant association between risk factors and acquisition of MRSA infection was seen in isolates that carried the SCCmec type III.
Conclusion: Presence of SCCmec types IV and V was confirmed by multiplex PCR in HA MRSA isolates which usually carry SCCmec types I, II and III only.
MRSA, Staphylococcus aureus, SCCmec types, HA MRSA, CA MRSA
ul Bashir Y, Bali N, Sharma S, Jahan T, Shafi H. SCCmec Type IV and V Methicillin Resistant Staphylococcus aureus Intrusion in Healthcare Settings. Am J Clin Microbiol Antimicrob. 2019; 2(1): 1032.